Gene name |
SPAC2C4.11c |
Gene ID |
09/F07 |
Gene synonyms/obsolete |
|
Gene product |
RNA-binding protein;
KH domain |
Entry clone |
Cloned |
ORF length (unspliced) |
777 |
ORF length (spliced) |
|
Entry clone length |
777 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
279T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC2C4.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGGCACCTACGGCAGC |
Rev primer name |
SPAC2C4.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATACGCTCTTTGGCACGA |
Amino acid length |
258 |
Molecular weight |
28.2 |
Isoelectric point (calc.) |
10.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYPPLVEHLLL/LLRLDDLYI |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |