Gene name |
SPCC1919.04 |
Gene ID |
09/E09 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
771 |
ORF length (spliced) |
|
Entry clone length |
771 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1919.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCTCTCGTTTATTGA |
Rev primer name |
SPCC1919.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGAATTTTTAATAGATCCC |
Amino acid length |
256 |
Molecular weight |
28.9 |
Isoelectric point (calc.) |
10.3 |
Signal SEQ |
|
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNYLLRSLKI/LRIVSLFLWI |
Localization (YFP) |
ER; cytoplasmic
dots |
Comments for localization |
large aggregates by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |