Gene name |
SPBC16C6.03c |
Gene ID |
09/E06 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
768 |
ORF length (spliced) |
477 |
Entry clone length |
768 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
550A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16C6.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCCTACCTCATTTAT |
Rev primer name |
SPBC16C6.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGATACAGGTTTAACAACC |
Amino acid length |
158 |
Molecular weight |
18 |
Isoelectric point (calc.) |
10.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |