| Gene name |
SPCC1672.02c |
| Gene ID |
09/E02 |
| Gene synonyms/obsolete |
sap1 |
| Gene product |
switch-activating
protein; required for efficient mat1 switching; essential;
N-terminal DNA-binding domain; C-terminal dimerization domain;
no apparent orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
765 |
| ORF length (spliced) |
|
| Entry clone length |
765 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1672.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGCTCCCAAGATGGA |
| Rev primer name |
SPCC1672.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGGTCACCAAGATTAGGA |
| Amino acid length |
254 |
| Molecular weight |
29.1 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus; nuclear
dots |
| Comments for localization |
fine dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |