Gene name |
SPCC1672.02c |
Gene ID |
09/E02 |
Gene synonyms/obsolete |
sap1 |
Gene product |
switch-activating
protein; required for efficient mat1 switching; essential;
N-terminal DNA-binding domain; C-terminal dimerization domain;
no apparent orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
765 |
ORF length (spliced) |
|
Entry clone length |
765 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1672.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGCTCCCAAGATGGA |
Rev primer name |
SPCC1672.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATGGTCACCAAGATTAGGA |
Amino acid length |
254 |
Molecular weight |
29.1 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
fine dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |