| Gene name |
SPBC2G5.02c |
| Gene ID |
09/D12 |
| Gene synonyms/obsolete |
|
| Gene product |
casein kinase II (beta
subunit); similar to Sp ckb1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
765 |
| ORF length (spliced) |
|
| Entry clone length |
765 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
38C:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2G5.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGCGAAAAAACCTCAT |
| Rev primer name |
SPBC2G5.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCACTTTCAGACTCCGAA |
| Amino acid length |
254 |
| Molecular weight |
29 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFNLTGLNL |
| Localization (YFP) |
nucleus>cytosol;
SPB? |
| Comments for localization |
nuclear dots by over
expression; 1~2 dots/nucleus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |