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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP27G11.07c
Gene ID 09/D10
Gene synonyms/obsolete
Gene product dual specificity protein kinase
Entry clone Cloned
ORF length (unspliced) 765
ORF length (spliced) 717
Entry clone length 765
No. of intron 1
Sequence status Finished
Sequence results 201A:T / 400T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP27G11.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGAAAAACCTGACCT
Rev primer name SPAP27G11.07.Rv
Rev primer SEQ AGAAAGCTGGGTATCCTATCATGGTACGTTTA
Amino acid length 238
Molecular weight 27.7
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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