Gene name |
SPAC16E8.02 |
Gene ID |
09/C04 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
754 |
ORF length (spliced) |
669 |
Entry clone length |
754 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
(-6)T:addition /
523T:C |
Comments |
5' terminus is
frameshifted. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC16E8.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTATGTAAGAATCTA |
Rev primer name |
SPAC16E8.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAACACAATACCATCGTCT |
Amino acid length |
222 |
Molecular weight |
24.9 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLLLTALVL/LLDNLIQSLFI/LSYCIRPLRL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |