Gene name |
SPBC1198.03c |
Gene ID |
09/B09 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
750 |
ORF length (spliced) |
|
Entry clone length |
750 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
715A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1198.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATATAAAAGATGAAAA |
Rev primer name |
SPBC1198.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAGGAAATAAGGAGTAAG |
Amino acid length |
249 |
Molecular weight |
28.2 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMGLDQILNL |
Localization (YFP) |
periphery at cell tip
and site of septum formation; cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |