Gene name |
SPCC550.08 |
Gene ID |
09/A09 |
Gene synonyms/obsolete |
|
Gene product |
N-acetyltransferase;
no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
744 |
ORF length (spliced) |
|
Entry clone length |
744 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
(-6)T:addition /
498T:C |
Comments |
5' terminus is
frameshifted. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC550.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGCTAAACGAGTTGA |
Rev primer name |
SPCC550.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCAAACAGGAGTAGATGAT |
Amino acid length |
247 |
Molecular weight |
28.2 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi?; ER?; vacuole
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |