| Gene name |
SPAC6F12.03c |
| Gene ID |
09/A05 |
| Gene synonyms/obsolete |
|
| Gene product |
SNARE; 1 t-SNARE
coiled-coil homology domain; involved in versicle-mediated
protein transport between the Golgi and the vacuole; no
apparent S. cerevisiae ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
744 |
| ORF length (spliced) |
|
| Entry clone length |
744 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
128T:C / 523A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6F12.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAATTTGCTTCTGAT |
| Rev primer name |
SPAC6F12.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGGAAGCCACAAGTAAT |
| Amino acid length |
247 |
| Molecular weight |
28.2 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |