| Gene name |
SPCC162.01c |
| Gene ID |
08/H08 |
| Gene synonyms/obsolete |
|
| Gene product |
nucleic acid binding
protein; similar to human RY-1; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
735 |
| ORF length (spliced) |
|
| Entry clone length |
735 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
195T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC162.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCGTCAAGAAGCGG |
| Rev primer name |
SPCC162.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCATTATCCAATGGACGA |
| Amino acid length |
244 |
| Molecular weight |
29.3 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
nuclear dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |