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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.04c
Gene ID 08/F09
Gene synonyms/obsolete gst3
Gene product glutathione S transferase
Entry clone Cloned
ORF length (unspliced) 729
ORF length (spliced)
Entry clone length 729
No. of intron 0
Sequence status Finished
Sequence results 209A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTGTTTTACACCACTT
Rev primer name SPAC688.04.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATCAGAGCGAACTTGC
Amino acid length 242
Molecular weight 28.1
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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