Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13A11.03
Gene ID 08/F07
Gene synonyms/obsolete
Gene product hypothetical coiled-coil protein; involved in DSB repair
Entry clone Cloned
ORF length (unspliced) 727
ORF length (spliced) 633
Entry clone length 727
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC13A11.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCCCAAGGGACTATC
Rev primer name SPAC13A11.03.Rv
Rev primer SEQ AGAAAGCTGGGTACAAAATCGGTAGTTGCAGA
Amino acid length 210
Molecular weight 24.2
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 10
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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