Gene name |
SPCC1259.06 |
Gene ID |
08/F05 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
725 |
ORF length (spliced) |
669 |
Entry clone length |
725 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1259.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGCCGTGATCGCGAA |
Rev primer name |
SPCC1259.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCTTTGGGAGCTCCATTTT |
Amino acid length |
222 |
Molecular weight |
25 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRNSFRELAL |
Localization (YFP) |
mitochondrion;
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |