| Gene name |
SPBC725.17c |
| Gene ID |
08/E05 |
| Gene synonyms/obsolete |
|
| Gene product |
RNA polymerase I
component |
| Entry clone |
Cloned |
| ORF length (unspliced) |
717 |
| ORF length (spliced) |
603 |
| Entry clone length |
717 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC725.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCTCTCCTTGTACCGT |
| Rev primer name |
SPBC725.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAATTCGACGTCAATTCCT |
| Amino acid length |
200 |
| Molecular weight |
23.1 |
| Isoelectric point (calc.) |
9.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCSLMHLCL |
| Localization (YFP) |
SPB;
nucleus>cytosol; periphery at site of septum formation;
faint spindle microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |