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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC576.02
Gene ID 08/D11
Gene synonyms/obsolete
Gene product hydantoin racemase; similar to Sp SPAC1F7.10 (paralog)
Entry clone Cloned
ORF length (unspliced) 711
ORF length (spliced)
Entry clone length 711
No. of intron 0
Sequence status Finished
Sequence results (-5)C:T / (-4)T:C / (-3)C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC576.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTACCATTCTCGTCAT
Rev primer name SPCC576.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGGATACGTATAGATGCCA
Amino acid length 236
Molecular weight 24.9
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization bright cytoplasmic dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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