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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC15E1.09
Gene ID 08/D09
Gene synonyms/obsolete grx2
Gene product arsenate reductase (glutaredoxin) activity; glutaredoxin; thioltransferase; similar to Sp grx1 (paralog)
Entry clone Cloned
ORF length (unspliced) 711
ORF length (spliced) 333
Entry clone length 711
No. of intron 3
Sequence status Finished
Sequence results 391T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC15E1.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTCTATAGCAAAAGC
Rev primer name SPAC15E1.09.Rv
Rev primer SEQ AGAAAGCTGGGTATAAAATTGAAGATTTGTTT
Amino acid length 110
Molecular weight 12.2
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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