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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16C4.17
Gene ID 08/D06
Gene synonyms/obsolete
Gene product hypothetical serine rich protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 708
ORF length (spliced)
Entry clone length 708
No. of intron 0
Sequence status Finished
Sequence results 5A:G / 18T:C / 300T:A / 445T:C / 599A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16C4.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACGTTTAGCTACTCG
Rev primer name SPCC16C4.17.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGCTATCGAATTCATCA
Amino acid length 235
Molecular weight 25.4
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>cytosol)
Microscope used for observation Confocal

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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