| Gene name |
SPBC23E6.06c |
| Gene ID |
08/C11 |
| Gene synonyms/obsolete |
|
| Gene product |
3,4-Dihydroxy-2-butanone 4-phosphate synthase
(putative); involved in riboflavin biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
706 |
| ORF length (spliced) |
615 |
| Entry clone length |
706 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
(-7)C:deletion /
(-2)A:deletion |
| Comments |
5' terminus is
frameshifted. |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC23E6.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTGCATCAATTGAGGA |
| Rev primer name |
SPBC23E6.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATACGCCCTTTAATGTAG |
| Amino acid length |
204 |
| Molecular weight |
22.5 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|