Gene name |
SPCC16A11.05c |
Gene ID |
08/B08 |
Gene synonyms/obsolete |
dim1 |
Gene product |
U4/U6.U5 snRNP;
involved in mRNA splicing; essential; required for
mitosis-possibly indirect |
Entry clone |
Cloned |
ORF length (unspliced) |
696 |
ORF length (spliced) |
429 |
Entry clone length |
696 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
299T:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC16A11.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTATTTTTTACCTCA |
Rev primer name |
SPCC16A11.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTAGCGATGTCGGGTAGAG |
Amino acid length |
142 |
Molecular weight |
16.9 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|