| Gene name |
SPCC16A11.05c |
| Gene ID |
08/B08 |
| Gene synonyms/obsolete |
dim1 |
| Gene product |
U4/U6.U5 snRNP;
involved in mRNA splicing; essential; required for
mitosis-possibly indirect |
| Entry clone |
Cloned |
| ORF length (unspliced) |
696 |
| ORF length (spliced) |
429 |
| Entry clone length |
696 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
299T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC16A11.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTATTTTTTACCTCA |
| Rev primer name |
SPCC16A11.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTAGCGATGTCGGGTAGAG |
| Amino acid length |
142 |
| Molecular weight |
16.9 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|