| Gene name |
SPCC16C4.03 |
| Gene ID |
07/G06 |
| Gene synonyms/obsolete |
pin1 |
| Gene product |
peptidyl-prolyl
cis-trans isomerase; WW domain which binds specifically to
phospho-Ser/Thr-Pro sequences; non-essential; overexpression
results in slow growth and G1 delay; functionally complements
Sc ESS1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
673 |
| ORF length (spliced) |
528 |
| Entry clone length |
673 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC16C4.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAATACTGGGTTACC |
| Rev primer name |
SPCC16C4.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCGTGTCTTTGAATGATG |
| Amino acid length |
175 |
| Molecular weight |
19.7 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |