| Gene name |
SPAC31G5.17c |
| Gene ID |
07/E09 |
| Gene synonyms/obsolete |
rps1001; rps10-1 |
| Gene product |
40S ribosomal protein
S10 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
654 |
| ORF length (spliced) |
435 |
| Entry clone length |
654 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC31G5.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTATTCCTAAGGAAAA |
| Rev primer name |
SPAC31G5.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGAGGACGACCGAAACCA |
| Amino acid length |
144 |
| Molecular weight |
16.2 |
| Isoelectric point (calc.) |
10.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus; nucleus>cytosol |
| Microscope used for
observation |
Zeiss,
DeltaVision |