| Gene name |
SPCC24E4.01 |
| Gene ID |
07/B01 |
| Gene synonyms/obsolete |
ade5; ade8;
SPCC569.08c |
| Gene product |
glycinamide
ribonucleotide transformylase; glycinamide ribonucleotide
transformylase; involved in purine biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
624 |
| ORF length (spliced) |
|
| Entry clone length |
624 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC24E4.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAGCTTCACTTGTCGT |
| Rev primer name |
SPCC24E4.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTATTGTCAGTAATGATC |
| Amino acid length |
207 |
| Molecular weight |
22.2 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |