| Gene name |
SPAC1296.04 |
| Gene ID |
06/G02 |
| Gene synonyms/obsolete |
|
| Gene product |
very hypothetical
protein; dysferlin-C terminal domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1142 |
| ORF length (spliced) |
747 |
| Entry clone length |
1142 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
ORF prediction was
changed. |
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1296.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGGAATCTGAAAGGAC |
| Rev primer name |
SPAC1296.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCTGGGGAGTTGGAGAT |
| Amino acid length |
248 |
| Molecular weight |
28.5 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
38/32 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery |
| Comments for localization |
a few cytoplasmic
dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |