Gene name |
SPCC622.18 |
Gene ID |
06/D11 |
Gene synonyms/obsolete |
rpl6 |
Gene product |
60S ribosomal protein
L6 |
Entry clone |
Cloned |
ORF length (unspliced) |
588 |
ORF length (spliced) |
|
Entry clone length |
588 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC622.18.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACAGTCAAGGTCAA |
Rev primer name |
SPCC622.18.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAACTTCATCAAGTGAGGA |
Amino acid length |
195 |
Molecular weight |
21.2 |
Isoelectric point (calc.) |
10.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|