| Gene name |
SPBC9B6.05c |
| Gene ID |
05/E12 |
| Gene synonyms/obsolete |
lsm3 |
| Gene product |
U6 snRNA-associated
protein; involved in mRNA splicing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
548 |
| ORF length (spliced) |
282 |
| Entry clone length |
548 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
402T:C / 444T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC9B6.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATCGGCACAGGCAGT |
| Rev primer name |
SPBC9B6.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTACGAGGTGGAGCAATC |
| Amino acid length |
93 |
| Molecular weight |
10.6 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus; one nuclear
dot |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |