| Gene name |
SPAC10F6.16 |
| Gene ID |
05/D01 |
| Gene synonyms/obsolete |
|
| Gene product |
cAMP-regulated
phosphoprotein/endosulfine conserved region; eukaryotic
conserved protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
529 |
| ORF length (spliced) |
420 |
| Entry clone length |
529 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
341T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC10F6.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGAACGCGCAAATG |
| Rev primer name |
SPAC10F6.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTCATTGCTTTCCAAG |
| Amino acid length |
139 |
| Molecular weight |
15.4 |
| Isoelectric point (calc.) |
10.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots;
nucleus>cytosol |
| Comments for localization |
aggregates |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |