Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16G5.04
Gene ID 05/A12
Gene synonyms/obsolete
Gene product mitochondrial ribosomal protein L23
Entry clone Cloned
ORF length (unspliced) 516
ORF length (spliced) 474
Entry clone length 516
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC16G5.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAACGTTGAATGGACA
Rev primer name SPBC16G5.04.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCGAGAATAATTCCTTC
Amino acid length 157
Molecular weight 17.7
Isoelectric point (calc.) 10.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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