Gene name |
SPAC23H3.02c |
Gene ID |
04/H12 |
Gene synonyms/obsolete |
|
Gene product |
transcription-splicing
co-factor; RING finger-like protein; involved in G2/M phase
transition (required); eukaryotic conserved protein; Sc
YPR094W is null lethal; UPF0123 domain |
Entry clone |
Cloned |
ORF length (unspliced) |
505 |
ORF length (spliced) |
354 |
Entry clone length |
505 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23H3.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAAGCGTATGGAAAT |
Rev primer name |
SPAC23H3.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATACGTTGCACCCGGCATT |
Amino acid length |
117 |
Molecular weight |
13.2 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |