| Gene name |
SPAC23H3.02c |
| Gene ID |
04/H12 |
| Gene synonyms/obsolete |
|
| Gene product |
transcription-splicing
co-factor; RING finger-like protein; involved in G2/M phase
transition (required); eukaryotic conserved protein; Sc
YPR094W is null lethal; UPF0123 domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
505 |
| ORF length (spliced) |
354 |
| Entry clone length |
505 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23H3.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAAGCGTATGGAAAT |
| Rev primer name |
SPAC23H3.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATACGTTGCACCCGGCATT |
| Amino acid length |
117 |
| Molecular weight |
13.2 |
| Isoelectric point (calc.) |
7.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |