Gene name |
SPCC16C4.13c |
Gene ID |
04/G12 |
Gene synonyms/obsolete |
rpl1201; rpl12-1;
rpl12.1 |
Gene product |
60S ribosomal protein
L12.1/L12A |
Entry clone |
Cloned |
ORF length (unspliced) |
498 |
ORF length (spliced) |
|
Entry clone length |
498 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC16C4.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCCTAAATTCGATCC |
Rev primer name |
SPCC16C4.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCTTGGGGGATCTCAATT |
Amino acid length |
165 |
Molecular weight |
17.6 |
Isoelectric point (calc.) |
10 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAPKIGPLGL |
Localization (YFP) |
cytosol;
nucleolus |
Comments for localization |
occasionally
nucleus>cytosol |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |