| Gene name |
SPAC8C9.17c |
| Gene ID |
04/G06 |
| Gene synonyms/obsolete |
|
| Gene product |
component of the DASH
complex, a microtubule-binding subcomplex of the outer
kinetochore that is essential for proper chromosome
segregation; the DASH complex mediates the formation and
maintenance of bipolar kinetochore-microtubule attachments by
forming closed rings around spindle microtubules and
establishing interactions with proteins from the central
kinetochore; non-essential; similar to S. cerevisiae
YKR037C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
495 |
| ORF length (spliced) |
|
| Entry clone length |
495 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
124T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC8C9.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGATTTGTTGAATTA |
| Rev primer name |
SPAC8C9.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGATTGTATCAGTTGCTTC |
| Amino acid length |
164 |
| Molecular weight |
19 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; dots on spindle
microtubules; nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |