Gene name |
SPAC12G12.05c |
Gene ID |
04/F10 |
Gene synonyms/obsolete |
|
Gene product |
transcription
initiation factor TFIID subunit; TAF(II) complex
(TBP-associated protein complex); SAGA complex; involved in
establishment and/or maintenance of chromatin
architecture |
Entry clone |
Cloned |
ORF length (unspliced) |
492 |
ORF length (spliced) |
|
Entry clone length |
492 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC12G12.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTCTCCAGGAATAAG |
Rev primer name |
SPAC12G12.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCAGACTTCACAGAGAAG |
Amino acid length |
163 |
Molecular weight |
18.4 |
Isoelectric point (calc.) |
5.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |