| Gene name |
SPBC31F10.02 |
| Gene ID |
04/E10 |
| Gene synonyms/obsolete |
|
| Gene product |
thioesterase
superfamily protein; human homolog is a novel candidate
oncogene MCT-1 involved in cell cycle progression and; has
multiple copies in T-cell malignancies; potential destruction
box (D-box); non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
486 |
| ORF length (spliced) |
|
| Entry clone length |
486 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
(-6)T:deletion |
| Comments |
5' terminus is
frameshifted. |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC31F10.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAATTAATAGCTCTGG |
| Rev primer name |
SPBC31F10.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTTCGGCTAAGTCAAGG |
| Amino acid length |
161 |
| Molecular weight |
17.1 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTDLGGSLAL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |