Gene name |
SPBC19G7.12c |
Gene ID |
04/B09 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan; overlaps with SPBC19G7.18c, one isn't
real |
Entry clone |
Cloned |
ORF length (unspliced) |
462 |
ORF length (spliced) |
|
Entry clone length |
462 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
Overlaps with
SPBC19G7.18c, one isn't real. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19G7.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAGGTTGCTCAGATT |
Rev primer name |
SPBC19G7.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGATTAGAAGTTCGGGAAGG |
Amino acid length |
153 |
Molecular weight |
18.3 |
Isoelectric point (calc.) |
10.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKHFLPELLI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |