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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC806.07
Gene ID 04/A08
Gene synonyms/obsolete ndk1
Gene product nucleoside diphosphate kinase; involved in CTP biosynthesis; involved in regulation of sexual development
Entry clone Cloned
ORF length (unspliced) 456
ORF length (spliced)
Entry clone length 456
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC806.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGACAGAACAAACTTT
Rev primer name SPAC806.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTCGTAAATCCAAGGTTCA
Amino acid length 151
Molecular weight 17
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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