Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4G9.06c
Gene ID 04/A02
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 453
ORF length (spliced) 342
Entry clone length 453
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4G9.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGAAGAACCGAAAAA
Rev primer name SPAC4G9.06.Rv
Rev primer SEQ AGAAAGCTGGGTACATAGTTTTATCTTGTGGT
Amino acid length 113
Molecular weight 13
Isoelectric point (calc.) 3.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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