Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1105.13c
Gene ID 03/E06
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; low correlation score; has transcript profile on microarray
Entry clone Cloned
ORF length (unspliced) 429
ORF length (spliced)
Entry clone length 429
No. of intron 0
Sequence status Finished
Sequence results (-15~-12):deletion / 347G:A
Comments 5' terminus is frameshifted; Abnormal attB1 sequence.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1105.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATCACGGAATTCATCAA
Rev primer name SPBC1105.13.Rv
Rev primer SEQ AGAAAGCTGGGTAATTCAACTTAACGAAGTGC
Amino acid length 142
Molecular weight 16.3
Isoelectric point (calc.) 5.8
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 4 images) See all images

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