| Gene name |
SPBC1105.13c |
| Gene ID |
03/E06 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan; low correlation score; has transcript profile
on microarray |
| Entry clone |
Cloned |
| ORF length (unspliced) |
429 |
| ORF length (spliced) |
|
| Entry clone length |
429 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
(-15~-12):deletion /
347G:A |
| Comments |
5' terminus is
frameshifted; Abnormal attB1 sequence. |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1105.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCACGGAATTCATCAA |
| Rev primer name |
SPBC1105.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTCAACTTAACGAAGTGC |
| Amino acid length |
142 |
| Molecular weight |
16.3 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |