Gene name |
SPCC1450.05c |
Gene ID |
03/D01 |
Gene synonyms/obsolete |
rox3 |
Gene product |
RNA polymerase II
holoenzyme and mediator subcomplex subunit |
Entry clone |
Cloned |
ORF length (unspliced) |
417 |
ORF length (spliced) |
|
Entry clone length |
417 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
251A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1450.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGCAAGCTCCTGAATA |
Rev primer name |
SPCC1450.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTCTTTCTACGACTTGGA |
Amino acid length |
138 |
Molecular weight |
15.9 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |