Gene name |
SPCC18.11c |
Gene ID |
03/C06 |
Gene synonyms/obsolete |
|
Gene product |
SET1 complex;
non-essential; Dpy-30 domain protein; involved in
transcriptional silencing; involved in regulation of chromatin
remodelling |
Entry clone |
Cloned |
ORF length (unspliced) |
414 |
ORF length (spliced) |
330 |
Entry clone length |
414 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
219A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC18.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAACAGCGCACCTGC |
Rev primer name |
SPCC18.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAAGCAGAATTTTTACCA |
Amino acid length |
109 |
Molecular weight |
11.8 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|