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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1834.04
Gene ID 03/B12
Gene synonyms/obsolete h3.1; hht1
Gene product histone H3; similar to Sp hht2 and hht3
Entry clone Cloned
ORF length (unspliced) 411
ORF length (spliced)
Entry clone length 411
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments Similar to SPBC8D2.04.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1834.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTCGTACTAAACAAAC
Rev primer name SPAC1834.04.Rv
Rev primer SEQ AGAAAGCTGGGTATGAGCGTTCGCCACGGAGA
Amino acid length 136
Molecular weight 15.3
Isoelectric point (calc.) 11.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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