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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC338.18
Gene ID 02/D02
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 354
ORF length (spliced)
Entry clone length 354
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC338.18.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGAACAGAATATCGA
Rev primer name SPCC338.18.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCAAACCGGAGGAATTA
Amino acid length 117
Molecular weight 13.3
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLGLISLAL/LNALLNRLRL
Localization (YFP) ER; Golgi?; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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