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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC622.05
Gene ID 02/B11
Gene synonyms/obsolete
Gene product very hypothetical protein
Entry clone Cloned
ORF length (unspliced) 348
ORF length (spliced)
Entry clone length 348
No. of intron 0
Sequence status Finished
Sequence results 10C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC622.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCGTTCTAAAGATGAT
Rev primer name SPCC622.05.Rv
Rev primer SEQ AGAAAGCTGGGTACATCCATTTATAGGAAGAA
Amino acid length 115
Molecular weight 13.5
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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