Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC27D7.05c
Gene ID 01/G03
Gene synonyms/obsolete apc14
Gene product anaphase-promoting complex (APC); involved in spore wall assembly; Mei4p dependent expression; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 324
ORF length (spliced)
Entry clone length 324
No. of intron 0
Sequence status Finished
Sequence results 15T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC27D7.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATCCGTTTGCTGGTAC
Rev primer name SPAC27D7.05.Rv
Rev primer SEQ AGAAAGCTGGGTACCATTCAGCCAACTGGTCA
Amino acid length 107
Molecular weight 12.1
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization a few moving dots; aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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