| Gene name |
SPAC23C4.11 |
| Gene ID |
01/C05 |
| Gene synonyms/obsolete |
atp18 |
| Gene product |
F-type ATPase subunit
j |
| Entry clone |
Cloned |
| ORF length (unspliced) |
276 |
| ORF length (spliced) |
183 |
| Entry clone length |
276 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23C4.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTTCTTTGGCCTTAA |
| Rev primer name |
SPAC23C4.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGATCACTTCCGTACTTT |
| Amino acid length |
60 |
| Molecular weight |
6.8 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |