| Gene name |
SPBC21B10.11 |
| Gene ID |
01/B12 |
| Gene synonyms/obsolete |
|
| Gene product |
dolichol phosphate
mannose regulatory protein (Dpm2-like); 1TM |
| Entry clone |
Cloned |
| ORF length (unspliced) |
262 |
| ORF length (spliced) |
219 |
| Entry clone length |
262 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
22G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC21B10.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGTTTATATCTCGAC |
| Rev primer name |
SPBC21B10.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAGTCCGACTTCTTTTTA |
| Amino acid length |
72 |
| Molecular weight |
8.2 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIGTFVSLLMI |
| Localization (YFP) |
ER; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss, DeltaVision
|